This study suggests that KV may be effective for delaying the development of clinical symptoms of influenza virus, and this may be through a mechanism unrelated to those deployed by the existing anti-influenza drugs but closely associated to its antioxidant and immunomodulatory properties. Figures - uploaded by Ifeoluwa Oluleke Awogbindin. Pretreatment with KV protects mice against H3N2 influenza virus.
Viral growth in the pulmonary tissue D was determined by hemagglutinating activity of lung homogenate with chicken red blood cells RBCsand lung weight was measured E as an index of pulmonary inflammation. Protection conferred by KV was measured using mean day of death and percentage protective index F.
Control animals were mock-treated with corn oil solution of DMSO. At 14 days post infection, surviving mice were euthanized and lungs harvested, rinsed, and placed in phosphate-buffered saline PBS to determine the degree of aeration.
KV improves survival of mice infected with H3N2 influenza virus. Lung weight E was measured as index of pulmonary inflammation, and pulmonary viral growth D was determined by hemagglutinating activity of lung homogenate with chicken RBC. Mice were euthanized and lungs harvested, rinsed, and placed in PBS to determine the degree of aeration. The unpredictability of broad-spectrum. This study presents the protective.
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These latter molecular bio. The test extract was prepared from Garcinia kola seeds. The seeds, obtained from Ile Ife, Nigeria, were peeled.
The extraction of KV from. Garcinia kola was done according to the method of Iwu. The extract was con.
The purity and identity of KV was deter. The of isolated. Ajanla Ibadan, Nigeriasubjected to hemagglutination as. Stock virus was aliquoted and. This study was carried out according to the guidelines and. H3N2 was instilled intranasally.
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For histopathology, a portion of the lungs was. The frozen lungs were later thawed, homogenized in. Viral growth in the pulmonary tissue D was determined by hemagglutinating. Protection conferred by KV was measured using mean day of death and percentage protective. Data are presented as mean — standard deviation SD of all.
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Myeloperoxidase MPO activity, an. Gross pathology via hydrostatic lungs test right panels and histopathological changes left of mice. At 14 days post infection, surviving mice were euthanized and lungs harvested, rinsed, and.
For histology, lung sections were stained. To determine the inhibitory prop erties of KV on. A total of 25 l Lo f0. Values of quantitative data were expressed as mean —. Comparison among groups was done by. The survival rate was analyzed by the Kaplan—.
KV improves morbidity while mortality onset is delayed. Though the virus was detected in the lungs of the treated. Figure 3 shows that the lungs of KV. With promising data in the previous study and the vin dication.
Compared with the infected but not treated mice with. Again, KV given 3 days prior conferred complete p rotection. Alveolar septa thickenin g and. In an attemp t to delineat e the underlyi ng mechanis m of. Since it was observ ed in the previous st udy that. KV may not miti gate viral repl ication but ma y impair mor. Consolid ation of the lu ngs in the KV-tre ated groups wa s.
Patholog ical examinat ions showed that KVat various. Gross pathol ogy via hydros tatic lungs test A a n d B and hi stopatholo gical change s— C a n d D Pozo de kola yahoo dating u n g sa n d E. Co ntrol animal s were mock-tre ated with corn oil so lution of DMSO. Mi ce were euthaniz ed and lungs. The li ver tissue s from the. However, li ver from. However, the GSH level was unaf. Oral administration of KV ameliorated the in.
In the liver, pretreatment with KV. Though the return to normality with. The levels of MDA, a. Further investigation revealed that KV may not be able to. The evolving IAV pandemic strain continues to outsmart. The implicated oxidative stress in.
After euthanasia, lungs and liver. This study inve stigated the prot ective effect of KVa bi. Garcinia ko la seed is a. Fl avonoid and poly phenols are.